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Goat anti-Rabbit IgG Heavy and Light Chain Human-Adsorbed Antibody Affinity Purified
Bethyl Laboratories
Catalog #
Target:
Rabbit IgG Heavy and Light Chain
Reactivity:
Rabbit
Applications:
ELISA
,ICC
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
HRP (0.5 mg)
Product Details
Specifications
Verified Reactivity
Rabbit
Antigen Species
Rabbit
Minimum Reactivity Species
Human
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Production Procedures
Antiserum was cross adsorbed using a human immunosorbent to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human IgG was detected. This antibody may cross react with IgG from other species.
Additional Product Information
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Applications
