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Goat anti-Human IgM-Fc Fragment Antibody Biotinylated
Bethyl Laboratories
Catalog #
Target:
Human IgM-Fc
Reactivity:
Human
Applications:
DD
,ELISA
,Flow Cyt
,ICC
,IEP
,IF
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin
,DyLight® 488
,DyLight® 550
,DyLight® 594
,DyLight® 650
,FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
,IgG Fraction
IgG Fraction (2 ml)
Unconjugated (1 mg)
Biotin (1 mg)
DyLight® 488 (0.5 mg)
DyLight® 550 (0.5 mg)
DyLight® 594 (0.5 mg)
DyLight® 650 (0.5 mg)
FITC (1 mg)
HRP (1 mg)
Product Details
Specifications
Verified Reactivity
Human
Antigen Species
Human
Concentration
1 mg/ml
0.5 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Production Procedures
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross reactivity with other immunoglobulins and light chains is less than 1%. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross reactivity with other immunoglobulins and light chains is less than 1%. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross reactivity with other immunoglobulins and light chains is less than 1%. This antibody may cross react with IgM from other species.
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross reactivity with other immunoglobulins and light chains is less than 1%. This antibody may cross react with IgM from other species.
Additional Product Information
IgM is found primarily in serum and is the first antibody made to fight infections. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Applications
