/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody [BLR091G]
Primary Antibodies  

Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody [BLR091G]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

VE-Cadherin

Reactivity:

Human

Applications:

ICC

,

IHC

,

IHC-IF

,

IP

,

WB

Platforms:

COMET™

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR091G

Format:

Whole IgG

Immunogen:

residues 48-599 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


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Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing the extracellular domain of human VE-Cadherin (residues 48-599, NP_001786.2).

Additional Product Information

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in adjacent cells. VE-Cadherin, or Cadherin-5 plays an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton and acts in concert with KRIT1 and MPP5 to establish and maintain correct endothelial cell polarity and vascular lumen. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P33151].

1003

CDH5

cadherin 5

P33151

Cadherin-5

Alternate Names

7B4; 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; CD antigen CD144; CD144; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; VE-cadherin

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.