/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-UBCH7 Antibody
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Primary Antibodies  

Rabbit anti-UBCH7 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (5)
Target:

UBCH7

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 100 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Bovine

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Ubiquitin conjugating enzyme H7 (UBCH7) is an E2 enzyme of the three step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ubiquitination provides an important post-translational means to regulate protein abundance and activity. UBCH7 has been shown to be involved in the ubiquitination of p53.

7332

UBE2L3

ubiquitin conjugating enzyme E2 L3

P68036

Ubiquitin-conjugating enzyme E2 L3

Alternate Names

E2 ubiquitin-conjugating enzyme L3; E2-F1; L-UBC; UBCH7; UbcM4; ubiquitin carrier protein L3; ubiquitin conjugating enzyme E2L 3; ubiquitin-conjugating enzyme E2 L3; ubiquitin-conjugating enzyme E2-F1; ubiquitin-conjugating enzyme UBCH7; ubiquitin-protein ligase L3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.