/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SIPA1 Antibody
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Primary Antibodies  

Rabbit anti-SIPA1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

SIPA1

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 992 and 1042

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

The regulation of GTPase activity is achieved via activators called GEFs (guanine-nucleotide exchange factors) and inactivators called GAPs (GTPase-activating proteins). Signal-induced proliferation-associated protein 1 (SIPA1) is a GTPase activating protein (GAP) for the Rap1 and Rap2 small GTPases. Spa-1 has been found to play an important role in hematopoietic cell development and the invasion and metastasis of human cancers.

6494

SIPA1

signal-induced proliferation-associated 1

Q96FS4

Signal-induced proliferation-associated protein 1

Alternate Names

GTPase-activating protein Spa-1; p130 SPA-1; signal-induced proliferation-associated gene 1; signal-induced proliferation-associated protein 1; sipa-1; SPA1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.