/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SHMT1 Antibody
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Primary Antibodies  

Rabbit anti-SHMT1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

SHMT1

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 453 and 483

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Serine hydroxymethyltransferase 1 (SHMT1) is the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6470)].

6470

SHMT1

serine hydroxymethyltransferase 1

P34896

Serine hydroxymethyltransferase, cytosolic

Alternate Names

CSHMT; cytoplasmic serine hydroxymethyltransferase; glycine hydroxymethyltransferase; serine hydroxymethyltransferase 1 (soluble); serine hydroxymethyltransferase, cytosolic; serine methylase; SHMT

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.