/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Septin 9 Antibody
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Primary Antibodies  

Rabbit anti-Septin 9 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

Septin 9

Reactivity:

Human

Applications:

IHC-IF

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 536 and 586

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to Septin 9 immobilized on solid support.

The epitope recognized by A302-354A-T maps to a region between residue 536 and 586 of human septin 9 using the numbering given in entry NP_001106963.1 (GeneID 10801).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to Septin 9 immobilized on solid support.

The epitope recognized by A302-354A maps to a region between residue 536 and 586 of human septin 9 using the numbering given in entry NP_001106963.1 (GeneID 10801).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Septin 9 is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia [taken from NCBI GeneID:10801].

10801

SEPT9

septin 9

Q9UHD8

Septin-9

Alternate Names

AF17q25; MLL septin-like fusion protein; MLL septin-like fusion protein MSF-A; MSF; MSF1; NAPB; Ov/Br septin; ovarian/breast septin; PNUTL4; SeptD1; septin D1; septin-9; SINT1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.