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Rabbit anti-RSK2 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

RSK2

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Guinea Pig,

Mouse,

Dog,

Pig,

Bovine,

Monkey

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to RSK2 immobilized on solid support.

The epitope recognized by IHC-00683 maps to a region between residue 1 and 50 of human ribosomal S6 kinase 2 using the numbering given in entry NP_004577.1 (GeneID 6197).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to RSK2 immobilized on solid support.

The epitope recognized by IHC-00683-T maps to a region between residue 1 and 50 of human ribosomal S6 kinase 2 using the numbering given in entry NP_004577.1 (GeneID 6197).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Ribosomal protein S6 kinase alpha-2 (RSK2) is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. RSK2 kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome [taken from NCBI Entrez Gene (GeneID: 6197)].

6197

RPS6KA3

ribosomal protein S6 kinase A3

P51812

Ribosomal protein S6 kinase alpha-3

Alternate Names

90 kDa ribosomal protein S6 kinase 3; CLS; epididymis secretory sperm binding protein; HU-3; insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK1B; MAPKAPK-1b; MRX19; p90-RSK 3; p90-RSK2; p90RSK3; pp90RSK2; ribosomal protein S6 kinase alpha-3; ribosomal protein S6 kinase, 90kDa, polypeptide 3; ribosomal S6 kinase 2; RSK; RSK2; RSK-2; S6K-alpha3; S6K-alpha-3

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.