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Primary Antibodies  

Rabbit anti-p115-RhoGEF Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (1)
Target:

p115-RhoGEF

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 775 and 825

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

P115-RhoGEF plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. P115-RhoGEF acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. The gene name for p115-RhoGEF is ARHGEF1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92888].

9138

ARHGEF1

Rho guanine nucleotide exchange factor 1

Q92888

Rho guanine nucleotide exchange factor 1

Alternate Names

115 kDa guanine nucleotide exchange factor; 115-kD protein; GEF1; LBCL2; LSC; Lsc homolog; p115RhoGEF; P115-RHOGEF; Rho guanine nucleotide exchange factor (GEF) 1; rho guanine nucleotide exchange factor 1; SUB1.5

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.