/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-NUPL Antibody
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Primary Antibodies  

Rabbit anti-NUPL Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

NUPL

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 512 and 562

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Bovine,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

NUPL or hRIP (human Rev interacting protein) was originally identified in a yeast two-hybrid screen for proteins that interact with the Rev protein of the human immunodeficiency virus type 1 (HIV-1). Structurally, hRIP contains an ARFGAP (ARF GTPase-activating protein) domain. hRIP was found to have homology with nucleoporins; thus it is also referred to as nucleoporin-like protein (NUPL) RIP. hRIP is essential to HIV-1 replication but not for cell viability, and it appears to be important for the export of HIV-1 RNA from the perinuclear region to the cytoplasm.

3267

AGFG1

ArfGAP with FG repeats 1

P52594

Arf-GAP domain and FG repeat-containing protein 1

Alternate Names

arf-GAP domain and FG repeat-containing protein 1; arf-GAP domain and FG repeats-containing protein 1; HIV-1 Rev-binding protein; HRB; hRIP, Rev interacting protein; nucleoporin-like protein RIP; RAB; Rab, Rev/Rex activation domain-binding protein; rev/Rex activation domain-binding protein; rev-interacting protein; RIP

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.