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Primary Antibodies  

Rabbit anti-NFAT5 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

NFAT5

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1481 and 1531

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

The product of the NFAT5 (TONEBP) gene, nuclear factor of activated T-cells 5, is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. NFAT5 regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, NFAT5 exists as a homodimer and forms stable dimers with DNA elements [taken from NCBI Entrez Gene (Gene ID: 10725)].

10725

NFAT5

nuclear factor of activated T cells 5

O94916

Nuclear factor of activated T-cells 5

Alternate Names

glutamine rich protein H65; NF-AT5; NFATL1; NFAT-like protein 1; NFAT; Znuclear factor of activated T-cells 5; nuclear factor of activated T-cells 5, tonicity-responsive; OREBP; osmotic response element-binding protein; T-cell transcription factor NFAT5; TonE-binding protein; TONEBP; tonicity-responsive enhancer-binding protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.