/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-NARG1 Antibody
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Primary Antibodies  

Rabbit anti-NARG1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

NARG1

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 816 and 866

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to NARG1 immobilized on solid support.

The epitope recognized by A302-147A maps to a region between residue 816 and 866 of human NMDA receptor regulated 1 using the numbering given in entry NP_476516.1 (GeneID 80155).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to NARG1 immobilized on solid support.

The epitope recognized by A302-147A-T maps to a region between residue 816 and 866 of human NMDA receptor regulated 1 using the numbering given in entry NP_476516.1 (GeneID 80155).

Additional Product Information

NARG1 (NMDA receptor-regulated 1) is also known as NATH (N-acetyltransferase). The NARG1 gene, along with NARG2 and NARG3, was originally identified as a gene regulated by NMDA receptors in developing neurons. The NARG1 gene was found to be a homolog of a yeast N-terminal acetyltransferase that functions in control of the G(0) phase of the cell cycle. NARG1 was also identified as NATH in experiments set out to identify genes differentially expressed in papillary thyroid carcinoma (PTC). The NATH protein has been shown to form a complex with human ARD1 where they function as an acetyltransferase and interact with ribosomal subunits. The ARD1/NATH complex plays an important role in cell survival.

80155

NAA15

N(alpha)-acetyltransferase 15, NatA auxiliary subunit

Q9BXJ9

N-alpha-acetyltransferase 15, NatA auxiliary subunit

Alternate Names

Ga19; gastric cancer antigen Ga19; MRD50; N-alpha-acetyltransferase 15, NatA auxiliary subunit; NARG1; NAT1P; NATH; NMDA receptor regulated 1; NMDA receptor-regulated protein 1; N-terminal acetyltransferase; protein tubedown-1; TBDN; TBDN100; transcriptional coactivator tubedown-100; tubedown-1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.