/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MED18 Antibody
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Primary Antibodies  

Rabbit anti-MED18 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (7)
Target:

MED18

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 150 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Zebrafish,

Bovine

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

MED18 is a component of the multisubunit mediator complex that functions as a bridge between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. MED18 forms a subcomplex with the MED8 and MED20 subunits and plays an important role in initiation complex formation.

54797

MED18

mediator complex subunit 18

Q9BUE0

Mediator of RNA polymerase II transcription subunit 18

Alternate Names

Mediator complex subunit 18; mediator of RNA polymerase II transcription subunit 18; mediator of RNA polymerase II transcription, subunit 18 homolog; p28b; SRB5; TRAP/mediator complex subunit p28b

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.