/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MED16 Antibody
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Primary Antibodies  

Rabbit anti-MED16 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (3)
Target:

MED16

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 550 and 600

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Mediator of RNA polymerase II transcription subunit 16 (MED16) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y2X0].

10025

MED16

mediator complex subunit 16

Q9Y2X0

Mediator of RNA polymerase II transcription subunit 16

Alternate Names

DRIP92; Mediator complex subunit 16; mediator of RNA polymerase II transcription subunit 16; THRAP5; thyroid hormone receptor-associated protein 5; thyroid hormone receptor-associated protein complex 95 kDa component; thyroid hormone receptor-associated protein, 95-kD subunit; TRAP95; vitamin D3 receptor-interacting protein complex 92 kDa component

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.