/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-HMGN1 Antibody
Primary Antibodies  

Rabbit anti-HMGN1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (3)
Target:

HMGN1

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 90 and 100

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to HMGN1 immobilized on solid support.

The epitope recognized by A302-363A maps to a region between residue 90 and 100 of human high-mobility group nucleosome binding domain 1 using the numbering given in entry NP_004956.5 (GeneID 3150).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to HMGN1 immobilized on solid support.

The epitope recognized by A302-363A-T maps to a region between residue 90 and 100 of human high-mobility group nucleosome binding domain 1 using the numbering given in entry NP_004956.5 (GeneID 3150).

Additional Product Information

HMGN1 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA to potentially alter the interaction between the DNA and the histone octamer. The two proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function [taken from NCBI Entrez Gene (GeneID:3150)].

3150

HMGN1

high mobility group nucleosome binding domain 1

P05114

Non-histone chromosomal protein HMG-14

Alternate Names

high mobility group nucleosome-binding domain-containing protein 1; high-mobility group (nonhistone chromosomal) protein 14; high-mobility group nucleosome binding 1; HMG14; non-histone chromosomal protein HMG-14; nonhistone chromosomal protein HMG-14

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.