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Rabbit anti-Histone H3 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

Histone H3

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 100 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

C. Elegans,

Chicken,

Pig,

Rabbit,

X. laevis,

X. tropicalis,

Zebrafish,

Bovine,

Rat,

D. melanogaster

Antigen Species

Human

Concentration
50 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to Histone H3 immobilized on solid support.

The epitope recognized by IHC-00465 maps to a region between residue 100 and the C-terminus (residue 135) of human Histone H3 using the numbering given in entry NP_003520.1 (GeneID 8350).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to Histone H3 immobilized on solid support.

The epitope recognized by IHC-00465-T maps to a region between residue 100 and the C-terminus (residue 135) of human Histone H3 using the numbering given in entry NP_003520.1 (GeneID 8350).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.

8350

HIST1H3A

histone cluster 1 H3 family member a

P68431

Histone H3.1

Alternate Names

H3 histone family, member A; H3/A; H3FA; histone 1, H3a; histone cluster 1, H3a; histone H3.1; histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

Applications

Epitope exposure is recommended.

Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.