/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-eIF2B5 Antibody
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Primary Antibodies  

Rabbit anti-eIF2B5 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (3)
Target:

eIF2B5

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 400 and 450

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

eIF2B5 is one of five subunits of the eukaryotic translation initiation factor 2B (eIF2B). eIF2B5 functions as a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in the EIF2B5 gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter (VWM) [taken from NCBI Entrez Gene (GeneID: 8893)].

8893

EIF2B5

eukaryotic translation initiation factor 2B subunit epsilon

Q13144

Translation initiation factor eIF-2B subunit epsilon

Alternate Names

CACH; CLE; EIF-2B; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2Bepsilon; eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa; LVWM; translation initiation factor eIF-2B subunit epsilon

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.