/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Cytokeratin 8 Recombinant Monoclonal Antibody [BLR299M]
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Primary Antibodies  

Rabbit anti-Cytokeratin 8 Recombinant Monoclonal Antibody [BLR299M]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

Cytokeratin 8

Reactivity:

Human

Applications:

Flow Cyt

,

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR299M

Format:

Whole IgG

Immunogen:

between 433 and 483 (C-term)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
50 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide, BSA-Free


Request Formulation Change

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Additional Product Information

Cytokeratin 8 is a member of the type II keratin family. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cytokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells where it plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. [taken from NCBI Entrez Gene (Gene ID: 3856)].

3856

KRT8

keratin 8

P05787

Keratin, type II cytoskeletal 8

Alternate Names

CARD2;CK8;CK-8;CYK8;cytokeratin-8;K2C8;K8;keratin 8, type II;keratin, type II cytoskeletal 8;Keratin-8;KO;type-II keratin Kb8

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.