/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-C3G Antibody
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Primary Antibodies  

Rabbit anti-C3G Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (5)
Target:

C3G

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 375 and 425

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

CRK SH3-binding GNRP (C3G) is a guanine nucleotide exchange factor (GEF) that binds to the SH3 domain of CRK and GRB2/ASH and catalyzes the exchange of GDP for GTP on GTPases of the Ras family. C3G has been reported to be involved in signaling that mediates cell adhesion and migration.

2889

RAPGEF1

Rap guanine nucleotide exchange factor 1

Q13905

Rap guanine nucleotide exchange factor 1

Alternate Names

C3G; CRK SH3-binding GNRP; GRF2; Guanine nucleotide-releasing factor 2; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene); Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; rap guanine nucleotide exchange factor 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.