/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Biotin Recombinant Monoclonal Antibody [BLR315M]
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Primary Antibodies  

Rabbit anti-Biotin Recombinant Monoclonal Antibody [BLR315M]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

Biotin

Applications:

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR315M

Format:

Whole IgG

Immunogen:

Biotin

Isotype:

IgG

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Concentration
250 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free


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Additional Product Information

Biotin, also known as Vitamin B7, is primarily known for its role in mammalian metabolism as a coenzyme. In molecular biology, biotin can be conjugated to molecules to serve as a binding partner for streptavidin.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.