/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Atg4B Antibody
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Primary Antibodies  

Rabbit anti-Atg4B Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

Atg4B

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 275 and 325

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Atg4B (ATG4 autophagy related 4 homolog B) is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9Y4P1].

23192

ATG4B

autophagy related 4B cysteine peptidase

Q9Y4P1

Cysteine protease ATG4B

Alternate Names

APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B; AUTL1; AUT-like 1 cysteine endopeptidase; autophagin-1; autophagy-related cysteine endopeptidase 1; autophagy-related protein 4 homolog B; cysteine protease ATG4B; hAPG4B

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.