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Rabbit anti-ARHGEF5 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

ARHGEF5

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 600 and 650

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ARHGEF5 immobilized on solid support.

The epitope recognized by IHC-00652 maps to a region between residue 600 and 650 of human Rho guanine nucleotide exchange factor (GEF) 5 using the numbering given in entry NP_005426.2 (GeneID 7984).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ARHGEF5 immobilized on solid support.

The epitope recognized by IHC-00652-T maps to a region between residue 600 and 650 of human Rho guanine nucleotide exchange factor (GEF) 5 using the numbering given in entry NP_005426.2 (GeneID 7984).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

ARHGEF5, also known as TIM (transforming immortalized mammary), is an oncogene that belongs to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho GTPases. It is implicated to play a role in proliferative breast disease.

7984

ARHGEF5

Rho guanine nucleotide exchange factor 5

Q12774

Rho guanine nucleotide exchange factor 5

Alternate Names

ephexin3; ephexin-3; GEF5; guanine nucleotide regulatory protein TIM; oncogene TIM; P60; p60 TIM; Rho guanine nucleotide exchange factor (GEF) 5; rho guanine nucleotide exchange factor 5; TIM; TIM1; transforming immortalized mammary oncogene

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.