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Rabbit anti-AKAP1 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

AKAP1

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 375 and 425

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to AKAP1 immobilized on solid support.

The epitope recognized by IHC-00281 maps to a region between residue 375 and 425 of human A kinase (PRKA) anchor protein 1 using the numbering given in entry NP_003479.1 (GeneID 8165).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to AKAP1 immobilized on solid support.

The epitope recognized by IHC-00281-T maps to a region between residue 375 and 425 of human A kinase (PRKA) anchor protein 1 using the numbering given in entry NP_003479.1 (GeneID 8165).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

AKAP1 (A-kinase anchor protein 1) was isolated as a protein that interacts with the regulatory subunit of protein kinase A (PKA) as well as the catalytic subunit of protein phosphatase 1 (PPI). AKAP1 plays a role in directing the subcellular localization of PKA to the cytoplasmic face of the outer mitochondrial membrane.

8165

AKAP1

A-kinase anchoring protein 1

Q92667

A-kinase anchor protein 1, mitochondrial

Alternate Names

A kinase (PRKA) anchor protein 1; AKAP; AKAP 149; AKAP121; AKAP149; AKAP84; A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 149 kDa; D-AKAP1; D-AKAP-1; Dual specificity A-kinase-anchoring protein 1; dual-specificity A-kinase anchoring protein 1; PPP1R43; PRKA1; protein kinase A anchoring protein 1; protein kinase A1; Protein kinase A-anchoring protein 1; protein phosphatase 1, regulatory subunit 43; SAKAP84; S-AKAP84; spermatid A-kinase anchor protein 84; TDRD17; testicular secretory protein Li 5; tudor domain containing 17

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.