/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ZEB2/SIP Recombinant Monoclonal Antibody [BLR115H]
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Primary Antibodies  

Rabbit anti-ZEB2/SIP Recombinant Monoclonal Antibody [BLR115H]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

ZEB2/SIP

Reactivity:

Human

Applications:

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR115H

Format:

Whole IgG

Immunogen:

between 1164 and 1214 (C-term)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
50 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change

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Additional Product Information

Smad interacting protein 1 (SIP1) is a transcriptional inhibitor that binds to the DNA sequence, 5'-CACCT-3', in different promoters. SIP1 has been shown to repress the transcription of E-cadherin. Defects in the gene which encodes for SIP1, ZEB2, are the cause of Hirschsprung disease-mental retardation syndrome (Hirschsprung disease) also known as Mowat-Wilson syndrome (MWS). Hirschsprung disease is a rare autosomal dominant complex developmental disorder [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60315].

9839

ZEB2

zinc finger E-box binding homeobox 2

O60315

Zinc finger E-box-binding homeobox 2

Alternate Names

HSPC082; SIP1; SIP-1; SMAD interacting protein 1; Smad-interacting protein 1; SMADIP1; ZFHX1B; zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; Zinc finger homeobox protein 1b

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.