/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CHD4 Recombinant Monoclonal Antibody [BLR066G]
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Primary Antibodies  

Rabbit anti-CHD4 Recombinant Monoclonal Antibody [BLR066G]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (1)
Target:

CHD4

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR066G

Format:

Whole IgG

Immunogen:

between residue 25 and 75

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
40 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

CHD4 is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4 can function as an activator of transcription in association with p300 histone acetyltransferase. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14839 and from NCBI Entrez Gene (Gene ID: 1108)].

1108

CHD4

chromodomain helicase DNA binding protein 4

Q14839

Chromodomain-helicase-DNA-binding protein 4

Alternate Names

ATP-dependent helicase CHD4; CHD-4; chromodomain-helicase-DNA-binding protein 4; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-BETA; SIHIWES

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.