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Goat anti-Mouse IgG2b Cross-Adsorbed Antibody FITC Conjugated
Bethyl Laboratories
Catalog #
Target:
Mouse IgG2b
Reactivity:
Mouse
Applications:
ELISA
,Flow Cyt
,ICC
,IF
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin
,FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
Biotin (0.5 mg)
FITC (0.5 mg)
HRP (0.5 mg)
Product Details
Specifications
Verified Reactivity
Mouse
Antigen Species
Mouse
Minimum Reactivity Species
Human, Rat
Concentration
0.5 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Production Procedures
Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG2b was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2b. Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG2b was detected. This antibody may cross react with IgG2b from other species.
Additional Product Information
IgG2b structure is more restricted than the traditional Y-shaped IgG2a molecule. 2b tends to be shaped more like a T as a result of intramolecular disulfide interactions.
Applications
