Detection of Human AKAP1 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-AKAP1 (Cat. No. IHC-00281) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).
Detection of Human AKAP8L/HA95 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-AKAP8L/HA95 (Cat. No. IHC-00277) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).
Detection of Human BRCA1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BRCA1 (Cat. No. IHC-00278) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).
Immunohistochemistry Accessory Kit (Cat. No. IHC-101) provides the reagents and method for sensitive, reliable and economical immunohistochemical staining. This Kit is designed for immunostaining formalin-fixed, paraffin-embedded tissue sections with primary antibodies made in rabbit. It contains all the reagents required for the immunostaining procedure and will stain 250 slides.
Detection of Human and Mouse PTPN11/SHP2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M, 50 mcg) cells. Antibodies: PTPN11/SHP2 antibody A301-544A used for WB at 0.04 mcg/ml (A) & 0.1 mcg/ml (B) & used for IP at 3 mcg/mg lysate. For blotting immunoprecipitated PTPN11/SHP2, ReliaBLOT® (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 second (B).
Detection of Human and Mouse STUB1/CHIP by Western Blot (h&m) & Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: STUB1/CHIP antibody A301-572A used for WB at 0.04 mcg/ml (A) and 1.0 mcg/ml (B) and used for IP at 3 mcg/mg lysate. STUB1/CHIP was also immunoprecipitated by STUB1/CHIP antibody A300-573A, which recognizes a downstream epitope. For blotting immunoprecipitated STUB1/CHIP, ReliaBLOT® (Cat. no. WB210) was used. Detection: Chemiluminescence with exposure times of 30 seconds (A&B).
Detection of Human PIP1/WDR84 by Western Blot & Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: PIP1/WDR84 antibody A301-550A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PIP1/WDR84 was also immunoprecipitated by PIP1/WDR84 antibody A301-549A, which recognizes an upstream epitope. For blotting immunoprecipitated PIP1/WDR84, ReliaBLOT® (Cat. No. WB120) was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
ReliaBLOT® IP/Western Blot Reagents and Procedures (patent pending) provide an improved method for the detection of immunoprecipitated proteins assayed via Western Blot. It seamlessly integrates into existing IP/WB Protocols with three simple changes. It is optimized for antibodies made in rabbit.
