Detection of Human cAbl by IHC-IF. Sample: FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-cAbl (Cat. No. IHC-00405) used at a dilution of 1:250. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.
Detection of Human BCR by IHC-IF. Sample: FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR (Cat. No. IHC-00406) used at a dilution of 1:250. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.
Detection of Human IKK-alpha by IHC. Sample: FFPE section of human ovarian tumor. Antibody: Affinity purified rabbit anti-IKK-alpha (Cat. No. IHC-00401) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).
Immunohistochemistry Accessory Kit (Cat. No. IHC-101) provides the reagents and method for sensitive, reliable and economical immunohistochemical staining. This Kit is designed for immunostaining formalin-fixed, paraffin-embedded tissue sections with primary antibodies made in rabbit. It contains all the reagents required for the immunostaining procedure and will stain 250 slides.
Detection of CKI epsilon by WB & IP. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: CKI epsilon antibody A302-135A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. CKI epsilon was also immunoprecipitated by a rabbit antibody (BL7891) directed to an epitope that is common to CKI epsilon and CKI delta. CKI delta was immunoprecipitated and blotted using A302-136A. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of CKI delta by WB & IP. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: CKI delta antibody A302-136A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. CKI delta was also immunoprecipitated by a rabbit antibody (BL7891) directed to an epitope that is common to CKI epsilon and CKI delta. CKI epsilon was immunoprecipitated and blotted using A302-135A. Detection: Chemiluminescence with exposure times of 30 seconds (A) & 3 seconds (B).
Detection of Human and Mouse TAB1 by WB (h&m) and IP (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Antibodies: TAB1 antibody A302-165A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. TAB1 was also immunoprecipitated by TAB1 antibody A302-166A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds (A) and 10 seconds (B).
ReliaBLOT® IP/Western Blot Reagents and Procedures (patent pending) provide an improved method for the detection of immunoprecipitated proteins assayed via Western Blot. It seamlessly integrates into existing IP/WB Protocols with three simple changes. It is optimized for antibodies made in rabbit.
