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Rabbit anti-USP47 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

USP47

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1237 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Zebra Finch,

Turkey,

Dog,

Chicken,

Pig,

Rabbit,

Bovine,

Monkey

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to USP47 immobilized on solid support.

The epitope recognized by IHC-00235 maps to a region between residue 1237 and the C-terminus (residue 1287) of human ubiquitin specific peptidase 47 using the numbering given in entry NP_060414.3 (GeneID 55031).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to USP47 immobilized on solid support.

The epitope recognized by IHC-00235-T maps to a region between residue 1237 and the C-terminus (residue 1287) of human ubiquitin specific peptidase 47 using the numbering given in entry NP_060414.3 (GeneID 55031).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase (USP47) is a UBP.

55031

USP47

ubiquitin specific peptidase 47

Q96K76

Ubiquitin carboxyl-terminal hydrolase 47

Alternate Names

deubiquitinating enzyme 47; Trf (TATA binding protein-related factor)-proximal homolog; TRFP; ubiquitin carboxyl-terminal hydrolase 47; ubiquitin thioesterase 47; ubiquitin thiolesterase 47; ubiquitin-specific-processing protease 47

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.