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Rabbit anti-USP3 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

USP3

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 471 and 521

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
100 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to USP3 immobilized on solid support.

The epitope recognized by IHC-00319 maps to a region between residue 471 and 521 of human ubiquitin specific protease 3 using the numbering given in entry AAD42992.1 (GeneID 9960).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to USP3 immobilized on solid support.

The epitope recognized by IHC-00319-T maps to a region between residue 471 and 521 of human ubiquitin specific protease 3 using the numbering given in entry AAD42992.1 (GeneID 9960).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 3 (USP3) is a member of this family and is involved in the deubiquitination of histones H2A and H2B.

9960

USP3

ubiquitin specific peptidase 3

Q9Y6I4

Ubiquitin carboxyl-terminal hydrolase 3

Alternate Names

deubiquitinating enzyme 3; SIH003; ubiquitin carboxyl-terminal hydrolase 3; ubiquitin thioesterase 3; ubiquitin thiolesterase 3; ubiquitin-specific-processing protease 3; UBP

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.