/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-UBF Antibody
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Primary Antibodies  

Rabbit anti-UBF Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

UBF

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 546 and 596

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

X. laevis,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Upstream binding factor (UBF) was originally identified as a multi-HMG (high mobility group)-box DNA binding domain protein that could enhance the in vitro assembly of the RNA polymerase I initiation complex. UBF is a transcription factor that recognizes the ribosomal RNA gene promoter required for the expression of 18S, 5.8S, and 28S ribosomal RNAs.

7343

UBTF

upstream binding transcription factor

P17480

Nucleolar transcription factor 1

Alternate Names

90-kDa nucleolus organizer region autoantigen; autoantigen NOR-90; CONDBA; NOR-90; nucleolar transcription factor 1; UBF; UBF1; UBF-1; UBF2; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.