/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-UBE4B Antibody
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Primary Antibodies  

Rabbit anti-UBE4B Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

UBE4B

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 125 and 175

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Ubiquitin conjugation factor E4 B (UBE4B) is an E4 enzyme of the multi-step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. The E4 enzyme is an additional conjugation factor which is involved in polyubiquitin chain assembly. UBE4B has been identified as a candidate neuroblastoma tumor suppressor gene.

10277

UBE4B

ubiquitination factor E4B

O95155

Ubiquitin conjugation factor E4 B

Alternate Names

E4; HDNB1; homologous to yeast UFD2; Homozygously deleted in neuroblastoma 1; homozygously deleted in neuroblastoma-1; RING-type E3 ubiquitin transferase E4 B; ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; ubiquitination factor E4B (UFD2 homolog, yeast); ubiquitin-fusion degradation protein 2; UBOX3; UFD2; UFD2A; UFD2A-III/UBE4B-III splice isoform

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.