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Rabbit anti-THRAP1/TRAP240 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

THRAP1/TRAP240

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 750 and 800

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

THRAP/TRAP240 is a component of the TRAP (thyroid hormone receptor associated protein) complex. The TRAP complex is also known as Mediator and functions as a coactivator of transcription by bridging DNA bound transcription factors to the basal transcription machinery. THRAP1/TRAP240 has been reported to form a complex with MED12, cyclin C, and CDK8 and negatively regulates transcription.

9969

MED13

mediator complex subunit 13

Q9UHV7

Mediator of RNA polymerase II transcription subunit 13

Alternate Names

activator-recruited cofactor 250 kDa component; ARC250; DRIP250; HSPC221; Mediator complex subunit 13; mediator of RNA polymerase II transcription subunit 13; mediator of RNA polymerase II transcription, subunit 13 homolog; THRAP1; thyroid hormone receptor-associated protein 1; thyroid hormone receptor-associated protein complex 240 kDa component; thyroid hormone receptor-associated protein complex component TRAP240; thyroid hormone receptor-associated protein, 240 kDa subunit; TRAP240; vitamin D3 receptor-interacting protein complex component DRIP250

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.