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Rabbit anti-SMG9 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

SMG9

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 75 and 125

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Guinea Pig,

Mouse,

Horse,

Dog,

Pig,

Rabbit,

Bovine,

Monkey,

Rat

Antigen Species

Human

Concentration
50 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to SMG9 immobilized on solid support.

The epitope recognized by IHC-00654 maps to a region between residue 75 and 125 of human suppressor with morphogenetic effect on genitalia protein 9 using the numbering given in entry NP_061981.2 (GeneID 56006).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to SMG9 immobilized on solid support.

The epitope recognized by IHC-00654-T maps to a region between residue 75 and 125 of human suppressor with morphogenetic effect on genitalia protein 9 using the numbering given in entry NP_061981.2 (GeneID 56006).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

SMG9 is a subunit of the SMG-1 complex. SMG-1 and Upf1 are part of a surveillance complex that participates in nonsense-mediated mRNA decay (NMD) to degrade mRNAs containing premature translation initiation codons (PTCs).

56006

SMG9

SMG9, nonsense mediated mRNA decay factor

Q9H0W8

Protein SMG9

Alternate Names

C19orf61; F17127_1; HBMS; protein SMG9; Protein SMG-9; protein smg-9 homolog

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.