/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SMC1 Recombinant Monoclonal Antibody [BL-205-2G8]
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Primary Antibodies  

Rabbit anti-SMC1 Recombinant Monoclonal Antibody [BL-205-2G8] (50 µg/ml)

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

SMC1

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

ICC

,

IHC

,

IHC-IF

,

IP

,

WB

Platforms:

PhenoImager™ HT

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BL-205-2G8

Format:

Whole IgG

Immunogen:

between 1200 to 1233 (C-term)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Bovine,

Rat

Antigen Species

Human

Concentration
50 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


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Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a synthetic peptide representing a region between residue 1200 and the C-terminus (residue 1233) of human structural maintenance of chromosomes 1 according to the numbering given in entry NP_006297.2 (GeneID 8243).

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a synthetic peptide representing a region between residue 1200 and the C-terminus (residue 1233) of human structural maintenance of chromosomes 1 according to the numbering given in entry NP_006297.2 (GeneID 8243).

Additional Product Information

Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.

8243

SMC1A

structural maintenance of chromosomes 1A

Q14683

Structural maintenance of chromosomes protein 1A

Alternate Names

CDLS2; DXS423E; epididymis secretory sperm binding protein; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC-1A; SMC1alpha; SMC-1-alpha; SMC1L1; SMCB; structural maintenance of chromosomes protein 1A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.