/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SAE2 Antibody
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Primary Antibodies  

Rabbit anti-SAE2 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (2)
Target:

SAE2

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 590 and 640

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

SAE2 (SUMO-activating enzyme subunit 2) is also known as UBA2 (ubiquitin-like modifier activating enzyme 2). Heterodimers of SAE1 and SAE2 act as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. Heterodimers mediate ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UBT2].

10054

UBA2

ubiquitin like modifier activating enzyme 2

Q9UBT2

SUMO-activating enzyme subunit 2

Alternate Names

anthracycline-associated resistance ARX; ARX; HRIHFB2115; SAE2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; SUMO-activating enzyme subunit 2; UBA2, ubiquitin-activating enzyme E1 homolog; ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.