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Rabbit anti-RPA43/TWISTNB Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

RPA43/TWISTNB

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 288 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to RPA43/TWISTNB immobilized on solid support.

The epitope recognized by A301-394A maps to a region between residue 288 and 338 of human DNA-directed RNA polymerase I subunit RPA43 (TWIST neighbor) using the numbering given in entry NP_001002926.1 (GeneID 221830).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to RPA43/TWISTNB immobilized on solid support.

The epitope recognized by A301-394A-T maps to a region between residue 288 and 338 of human DNA-directed RNA polymerase I subunit RPA43 (TWIST neighbor) using the numbering given in entry NP_001002926.1 (GeneID 221830).

Additional Product Information

RPA43 is a component of the RNA polymerase I complex which synthesizes ribosomal RNA precursors.

221830

TWISTNB

TWIST neighbor

Q3B726

DNA-directed RNA polymerase I subunit RPA43

Alternate Names

DNA-directed RNA polymerase I subunit RPA43; twist neighbor protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).