/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-RNF25 Antibody
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Primary Antibodies  

Rabbit anti-RNF25 Antibody Affinity Purified

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

RNF25

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 409 and 459

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

RNF25 (RING finger protein 25) is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (by similarity). RNF25 stimulates transcription mediated by NF-kappa-B [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96BH1].

64320

RNF25

ring finger protein 25

Q96BH1

E3 ubiquitin-protein ligase RNF25

Alternate Names

AO7; E3 ubiquitin-protein ligase RNF25; RING finger protein 25; ring finger protein AO7; RING-type E3 ubiquitin transferase RNF25

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.