/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-PHGDH Antibody
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Primary Antibodies  

Rabbit anti-PHGDH Antibody Affinity Purified

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (4)
Target:

PHGDH

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Pig,

Bovine

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

D-3-phosphoglycerate dehydrogenase (PHGDH) is an enzyme involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptom [taken from NCBI Entrez Gene (Gene ID: 26227)].

26227

PHGDH

phosphoglycerate dehydrogenase

O43175

D-3-phosphoglycerate dehydrogenase

Alternate Names

2-oxoglutarate reductase; 3PGDH; 3-PGDH; 3-phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; HEL-S-113; malate dehydrogenase; NLS; NLS1; PDG; PGAD; PGD; PGDH; PHGDHD; SERA

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.