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Rabbit anti-PBE Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

PBE

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 673 and 723

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Peroxisomal bifunctional enzyme (PBE) is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of PBE contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in the PBE gene are a cause of peroxisomal disorders such as Zellweger syndrome [taken from NCBI Entrez Gene (Gene ID: 1962)].

1962

EHHADH

enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase

Q08426

Peroxisomal bifunctional enzyme

Alternate Names

3,2-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; ECHD; Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; FRTS3; L-3-hydroxyacyl-CoA dehydrogenase; LBFP; L-bifunctional protein, peroxisomal; LBP; L-PBE; PBE; PBFE; peroxisomal bifunctional enzyme; peroxisomal enoyl-CoA hydratase

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.