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Rabbit anti-NUCKS IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

NUCKS

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Guinea Pig,

Horse,

Monkey

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to NUCKS immobilized on solid support.

The epitope recognized by IHC-00303 maps to a region between residue 1 and 50 of human nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate using the numbering given in entry NP_073568.2 (GeneID 64710).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to NUCKS immobilized on solid support.

The epitope recognized by IHC-00303-T maps to a region between residue 1 and 50 of human nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate using the numbering given in entry NP_073568.2 (GeneID 64710).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene.

64710

NUCKS1

nuclear casein kinase and cyclin dependent kinase substrate 1

Q9H1E3

Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1

Alternate Names

JC7; NUCKS; nuclear ubiquitous casein and cyclin-dependent kinase substrate 1; nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate; P1; potential LAG1 interactor

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.