/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-HCS Antibody
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Primary Antibodies  

Rabbit anti-HCS Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

HCS

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Holocarboxylase synthase (HCS) is an enzyme that catalyzes the binding of biotin to carboxylases and histones. HCS plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism [taken from NCBI Entrez Gene (Gene ID: 3141)].

3141

HLCS

holocarboxylase synthetase

P50747

Biotin--protein ligase

Alternate Names

biotin apo-protein ligase; biotin--acetyl-CoA-carboxylase ligase; biotin--methylcrotonoyl-CoA-carboxylase ligase; biotin--methylmalonyl-CoA-carboxytransferase ligase; biotin--protein ligase; HCS; Holocarboxylase synthetase; holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase); holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.