/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-GRAF Antibody
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Primary Antibodies  

Rabbit anti-GRAF Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

GRAF

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 611 and 661

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. GTPase regulator associated with focal adhesion kinase (GRAF) is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc4 [taken from NCBI Entrez Gene (Gene ID: 23092)].

23092

ARHGAP26

Rho GTPase activating protein 26

Q9UNA1

Rho GTPase-activating protein 26

Alternate Names

GRAF; GRAF1; GTPase regulator associated with focal adhesion kinase; GTPase regulator associated with focal adhesion kinase pp125(FAK); oligophrenin-1-like protein; OPHN1L; OPHN1L1; rho GTPase-activating protein 26; Rho-type GTPase-activating protein 26

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.