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Rabbit anti-GCC185 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

GCC185

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

185 kDa Golgi coiled-coil protein (GCC85) is a Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector, GCC85 is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. Golgin may also play a role in transport between the recycling endosomes and the Golgi. It is required for maintenance of the Golgi structure, and it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2. RanBP2L4, renal carcinoma antigen NY-REN-53, and KIAA0336 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q81WJ2].

9648

GCC2

GRIP and coiled-coil domain containing 2

Q8IWJ2

GRIP and coiled-coil domain-containing protein 2

Alternate Names

185 kDa Golgi coiled-coil protein; CLL-associated antigen KW-11; CTCL tumor antigen se1-1; GCC protein, 185-kD; GCC185; Golgi coiled-coil protein GCC185; GRIP and coiled-coil domain-containing protein 2; Ran-binding protein 2-like 4; RANBP2L4; REN53; renal carcinoma antigen NY-REN-53

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.