/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ETO Antibody
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Primary Antibodies  

Rabbit anti-ETO Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

ETO

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 554 and 604

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

ETO (eight twenty-one protein) is also known as MTG8 (myeloid translocation gene on 8q22) and was identified as part of an 8:21 chromosomal breakpoint in leukemia cells. ETO is encoded by the RUNX1T1 (runt-related transcription factor; translocated to, 1 (cyclin D-related) gene and functions as a transcriptional co-repressor.

862

RUNX1T1

RUNX1 translocation partner 1

Q06455

Protein CBFA2T1

Alternate Names

acute myelogenous leukemia 1 translocation 1, cyclin-D related; AML1-MTG8; AML1T1; CBFA2T1; CDR; core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related; Cyclin-D-related protein; eight twenty one protein; ETO; MTG8; myeloid translocation gene on 8q22; protein CBFA2T1; Protein ETO; Protein MTG8; runt related transcription factor 1; translocated to, 1 (cyclin D related); zinc finger MYND domain-containing protein 2; ZMYND2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.