/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ENT1 Antibody
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Primary Antibodies  

Rabbit anti-ENT1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

ENT1

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 343 and 393

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Equilibrative nucleoside transporter 1 (ENT1) mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine and is inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q99808].

2030

SLC29A1

solute carrier family 29 member 1 (Augustine blood group)

Q99808

Equilibrative nucleoside transporter 1

Alternate Names

ENT1; Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; equilibrative nucleoside transporter 1; nucleoside transporter, es-type; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.