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Rabbit anti-DEAF1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

DEAF1

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 515 and 565

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Deformed epidermal autoregulatory factor 1 homolog (DEAF1) is a transcription factor that binds to sequences with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene and also binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. DEAF1 regulates epithelial cell proliferation and side-branching in the mammary gland and also controls the expression of peripheral tissue antigens in pancreatic lymph nodes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O75398].

10522

DEAF1

DEAF1 transcription factor

O75398

Deformed epidermal autoregulatory factor 1 homolog

Alternate Names

deformed epidermal autoregulatory factor 1 homolog; MRD24; nuclear DEAF-1-related transcriptional regulator; NUDR; SPN; suppressin; zinc finger MYND domain-containing protein 5; ZMYND5

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.