/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CDC6 Antibody
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Primary Antibodies  

Rabbit anti-CDC6 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CDC6

Reactivity:

Human

Applications:

IHC

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Cell division cycle protein 6 homolog (CDC6) is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during the cell cycle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins [taken from NCBI Entrez Gene (GeneID: 990)].

990

CDC6

cell division cycle 6

Q99741

Cell division control protein 6 homolog

Alternate Names

CDC18L; cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; cell division control protein 6 homolog; cell division cycle 6 homolog; HsCDC18; HsCDC6; MGORS5; p62(cdc6)

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.