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Rabbit anti-BAD IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

BAD

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Cat,

Dog,

Monkey,

Rat

Antigen Species

Human

Concentration
100 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to BAD immobilized on solid support.

The epitope recognized by IHC-00677 maps to a region between residue 1 and 50 of human BCL2-antagonist of cell death using the numbering given in entry NP_004313.1 (GeneID 572).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to BAD immobilized on solid support.

The epitope recognized by IHC-00677-T maps to a region between residue 1 and 50 of human BCL2-antagonist of cell death using the numbering given in entry NP_004313.1 (GeneID 572).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

BAD (Bcl2 agonist of cell death) is a member of the Bcl-2 family. Bcl-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein [taken from NCBI Entrez Gene (GeneID:572)].

572

BAD

BCL2 associated agonist of cell death

Q92934

Bcl2-associated agonist of cell death

Alternate Names

BAD; BBC2; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; bcl2-associated agonist of cell death; bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; bcl2-L-8; bcl-2-like protein 8; BCL-X/BCL-2 binding protein; bcl-XL/Bcl-2-associated death promoter

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.