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Rabbit anti-YAP1 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

YAP1

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 350 and 400

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Horse,

Sheep,

Dog,

Pig,

Bovine,

Monkey

Antigen Species

Human

Concentration
100 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to YAP1 immobilized on solid support.

The epitope recognized by IHC-00665 maps to a region between residue 350 and 400 of human Yes-associated protein 1 using the numbering given in entry NP_006097.1 (GeneID 10413).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to YAP1 immobilized on solid support.

The epitope recognized by IHC-00665-T maps to a region between residue 350 and 400 of human Yes-associated protein 1 using the numbering given in entry NP_006097.1 (GeneID 10413).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Yes-associated protein 1 (YAP1) is the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction [taken from NCBI GeneID:10413].

10413

YAP1

Yes associated protein 1

P46937

Transcriptional coactivator YAP1

Alternate Names

65 kDa Yes-associated protein; COB1; protein yorkie homolog; transcriptional coactivator YAP1; YAP; YAP2; YAP65; yes-associated protein 1; yes-associated protein 2; yes-associated protein YAP65 homolog; YKI; yorkie homolog

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.