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Rabbit anti-eIF3B/EIF3S9 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

eIF3B/EIF3S9

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 100 and 150

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
100 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to eIF3B/EIF3S9 immobilized on solid support.

The epitope recognized by IHC-00659 maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 3, subunit B (eukaryotic translation initiation factor 3, subunit 9) using the numbering given in entry NP_003742.2 (GeneID 8662).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to eIF3B/EIF3S9 immobilized on solid support.

The epitope recognized by IHC-00659-T maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 3, subunit B (eukaryotic translation initiation factor 3, subunit 9) using the numbering given in entry NP_003742.2 (GeneID 8662).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.

8662

EIF3B

eukaryotic translation initiation factor 3 subunit B

P55884

Eukaryotic translation initiation factor 3 subunit B

Alternate Names

eIF3 p110; eIF3 p116; eIF3b; eIF-3-eta; EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; hPrt1; PRT1; prt1 homolog

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.